Collab with Nature Microbiology Magazine
Illustration shown in the front cover of Nature Microbiology for the Issue 1, Volume 5 titled “Phage protection in a (nut)shell”.
The project present the final cover and an additional optional cover that could be presented in future issues. Both illustrate the focus researched in the main paper, the case of a jumbo phage that forms a nucleus-like structure evading CRISPR–Cas.
The project present the final cover and an additional optional cover that could be presented in future issues. Both illustrate the focus researched in the main paper, the case of a jumbo phage that forms a nucleus-like structure evading CRISPR–Cas.
The main issue was presented in the online web of the magazine https://www.nature.com/nmicrobiol/volumes/5/issues/1
Abstract
CRISPR–Cas systems provide bacteria with adaptive immunity against bacteriophages1. However, DNA modification2,3, the production of anti-CRISPR proteins4,5 and potentially other strategies enable phages to evade CRISPR–Cas. Here, we discovered a Serratia jumbo phage that evades type I CRISPR–Cas systems, but is sensitive to type III immunity. Jumbo phage infection resulted in a nucleus-like structure enclosed by a proteinaceous phage shell—a phenomenon only reported recently for distantly related Pseudomonas phages6,7. All three native CRISPR–Cas complexes in Serratia—type I-E, I-F and III-A—were spatially excluded from the phage nucleus and phage DNA was not targeted.
